FAQ

Cells:

Cells need to be washed 3 times in cold PBS in a centrifuge tube (this removes condition media that contains bovine growth factors), all PBS needs to be removed and the cell pellet is then snap frozen in liquid nitrogen and transferred to -80°C for storage.

If lysis was done please indicate what lysis buffer was used.

Samples should be transported to us on dry ice.

200,000 cells should be enough for regular proteomic profiling.

If a PTM enrichment is planned such as phosphor-proteome study, a minimum of 10^7 is required for optimal results, although we could attempt an enrichment with less.

Tissues:

Tissues should be quickly washed with cold PBS, to remove blood, then snap frozen in liquid nitrogen and transferred to -80°C for storage.

Samples should be transported to us on dry ice.

The sample will be homogenized in the protein center in its entirety, so samples should be sent in a manner that doesn’t need further dissection.

Paraffin-embedded (FFPE) block of tissue should be sliced (10micron slices). Please transfer the area of interest of the slice to an Eppendorf tube. Usually 2 slices are sufficient. Samples can be sent in RT.

Plants:

Leaves should either be sent to us whole, we then pulverize them in liquid nitrogen to create a powder. Or sent already in a powder form.

If RuBisCO is very abundant in the plant, we recommend performing a RuBisCO depletion in the Protein center.

Exosomes/vesicles

Purified exosomes can be sent in minimal volume in PBS or in 2%SDS with 100mM Tris.

Samples should be transported to us on dry ice.

Co-IP or Bio-ID

Please contact us while you are designing the experiment for consultation.

Gels:

Homemade gels need to be cast at least a day ahead, for full gel polymerization.
Commercial pre-cast gels are ready to use.

After gel staining, gels should be destained for at least 10min, even if regular recommended staining is in water. This is to prevent formation of mold while the gel is stored before processing. After destaining, a picture should be taken, and marked with the sample names and areas of interest.

If the entire lane is needed for proteomic processing, please run for only a 2-3 cm of the lane. If only specific slices are needed, run the entire lane.

You can send us the gel whole, with instructions on the lanes/slices/bands of interest, or already cut and placed in a centrifuge tube.

Store in 4°C until delivery. Samples can be sent in RT.

Please fill in the sample information form.

Please sent us a request for a price quotation.

You are welcome to send your samples Sunday to Thursday 8-16.

Please advise that your samples are on the way.

https://www.proteomexchange.org/submission/index.html

Please let us know if you need help or additional files.